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Western blot analysis of extracts of various cell lines, using XIAP antibody (A6869) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (A6869) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded human kidney using XIAP antibody (A6869) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using XIAP antibody (A6869) at dilution of 1:100 (40x lens).
[KO Validated] XIAP Rabbit pAb
Catalog No：AB-A6869
Manufacturer：ABclonal
Synonyms：XIAP;API3;BIRC4;IAP-3;ILP1;MIHA;XLP2;hIAP-3;hIAP3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] XIAP Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2). |
| Gene ID: | 331 |
| Swiss prot: | P98170 |
| Molecular weight: | 56kDa |
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