Western blot analysis of extracts from normal (control) and USP14 knockout (KO) HeLa cells, using USP14 antibody (A19998) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] USP14 Rabbit pAb
Catalog No:AB-A19998
Manufacturer:ABclonal
Synonyms:USP14;TGT
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] USP14 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Immunogen: | A synthetic peptide of human USP14 |
Gene ID: | 9097 |
Swiss prot: | P54578 |
Molecular weight: | 52kDa/54kDa/56kDa |
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