Western blot analysis of extracts of various cell lines, using TLK2 antibody (A6770) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and TLK2 Rabbit pAb knockout (KO) NIH/3T3 cells, using TLK2 Rabbit pAb antibody (A6770) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
[KO Validated] TLK2 Rabbit pAb
Catalog No:AB-A6770
Manufacturer:ABclonal
Synonyms:TLK2;HsHPK;PKU-ALPHA
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] TLK2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2). |
Gene ID: | 11011 |
Swiss prot: | Q86UE8 |
Molecular weight: | 82kDa/85kDa/87kDa |
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