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Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A11483) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts from normal (control) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody (A11483) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded Rat ovary using SQSTM1/p62 antibody (A11483) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human appendix using SQSTM1/p62 antibody (A11483) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse liver using SQSTM1/p62 antibody (A11483) at dilution of 1:100 (40x lens).
[KO Validated] SQSTM1/p62 Rabbit pAb
Catalog No：AB-A11483
Manufacturer：ABclonal
Synonyms：SQSTM1;A170;DMRV;FTDALS3;NADGP;OSIL;PDB3;ZIP3;p60;p62;p62B
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] SQSTM1/p62 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_001135770.1). |
| Gene ID: | 8878 |
| Swiss prot: | Q13501 |
| Molecular weight: | 38kDa/47kDa |
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