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Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts from normal (control) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (A19115) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of various cell lines, using Smad3 antibody (A19115) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
[KO Validated] Smad3 Rabbit mAb
Catalog No：AB-A19115
Manufacturer：ABclonal
Synonyms：HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3;SMAD3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] Smad3 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009] |
| Immunogen: | A synthesized peptide derived from human Smad3 |
| Gene ID: | 4088 |
| Swiss prot: | P84022 |
| Molecular weight: | 58kDa |
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