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Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (A5718) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (A5718) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Western blot analysis of extracts of 293T cells, using [KO Validated] SIRT3 antibody (A5718) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human thyroid cancer using SIRT3 antibody (A5718) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (A5718) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SIRT3 antibody (A5718) at dilution of 1:100 (40x lens).
[KO Validated] SIRT3 Rabbit pAb
Catalog No：AB-A5718
Manufacturer：ABclonal
Synonyms：SIRT3;SIR2L3;sirtuin 3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] SIRT3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1). |
| Gene ID: | 23410 |
| Swiss prot: | Q9NTG7 |
| Molecular weight: | 28kDa/43kDa |
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