Western blot analysis of extracts of various cell lines, using SIRT1 antibody (A11267) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (A11267) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] SIRT1 Rabbit pAb
Catalog No:AB-A11267
Manufacturer:ABclonal
Synonyms:SIR2;SIR2L1;SIR2alpha;SIRT1;sirtuin 1
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] SIRT1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2). |
Gene ID: | 23411 |
Swiss prot: | Q96EB6 |
Molecular weight: | 61kDa/81kDa |
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