Western blot analysis of extracts from normal (control) and RING1B/RNF2 knockout (KO) HeLa cells, using RING1B/RNF2 antibody (A18076) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
[KO Validated] RING1B/RNF2 Rabbit pAb
Catalog No:AB-A18076
Manufacturer:ABclonal
Synonyms:RNF2;BAP-1;BAP1;DING;HIPI3;RING1B;RING2
Applications:WB,IHC,IF,IP,ChIP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] RING1B/RNF2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1). |
Gene ID: | 6045 |
Swiss prot: | Q99496 |
Molecular weight: | 29kDa/37kDa |
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