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Western blot analysis of extracts of various cell lines, using RB antibody (A0003) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and RB Rabbit pAb knockout (KO) HeLa cells, using RB Rabbit pAb antibody (A0003) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunofluorescence analysis of Y79 cells using Rb Polyclonal Antibody (A0003) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts from normal (control) and RB Rabbit pAb knockout (KO) HeLa cells, using RB Rabbit pAb antibody (A0003) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] RB Rabbit pAb
Catalog No：AB-A0003
Manufacturer：ABclonal
Synonyms：OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] RB Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 701-928 of human RB (NP_000312.2). |
| Gene ID: | 5925 |
| Swiss prot: | P06400 |
| Molecular weight: | 106kDa |
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