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Western blot analysis of extracts from normal (control) and RB knockout (KO) HeLa cells, using RB antibody (A3618) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] RB Rabbit mAb
Catalog No：AB-A3618
Manufacturer：ABclonal
Synonyms：OSRC, PPP1R130, RB, p105-Rb, pRb, pp110
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] RB Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human RB |
| Gene ID: | 5925 |
| Swiss prot: | P06400 |
| Molecular weight: | 105kDa |
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