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Western blot analysis of extracts of various cell lines, using PTEN antibody (A19104) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Western blot analysis of extracts from normal (control) and PTEN knockout (KO) HeLa cells, using PTEN antibody (A19104) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] PTEN Rabbit mAb (A19104) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb (A19104) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
[KO Validated] PTEN Rabbit mAb
Catalog No：AB-A19104
Manufacturer：ABclonal
Synonyms：10q23del;BZS;CWS1;DEC;GLM2;MHAM;MMAC1;PTEN1;TEP1;PTEN;PTENbeta
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] PTEN Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015] |
| Immunogen: | A synthesized peptide derived from human PTEN |
| Gene ID: | 5728 |
| Swiss prot: | P60484 |
| Molecular weight: | 54kDa |
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