Western blot analysis of extracts of various cell lines, using PRMT6 antibody (A7814) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and PRMT6 Rabbit pAb knockout (KO) 293T cells, using PRMT6 Rabbit pAb antibody (A7814) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] PRMT6 Rabbit pAb
Catalog No:AB-A7814
Manufacturer:ABclonal
Synonyms:PRMT6;HRMT1L6
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] PRMT6 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human PRMT6 (NP_060607.2). |
Gene ID: | 55170 |
Swiss prot: | Q96LA8 |
Molecular weight: | 32kDa/41kDa |
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