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Western blot analysis of extracts of various cell lines, using PKR/PKR/EIF2AK2 antibody (A5578) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human liver using PKR/PKR/EIF2AK2 antibody (A5578) at dilution of 1:100 (40x lens).
[KO Validated] PKR/EIF2AK2 Rabbit pAb
Catalog No：AB-A5578
Manufacturer：ABclonal
Synonyms：EIF2AK1;PKR;PPP1R83;PRKR;Eif2ak2;EIF2AK2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] PKR/EIF2AK2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PKR/PKR/EIF2AK2 (NP_002750.1). |
| Gene ID: | 5610 |
| Swiss prot: | P19525 |
| Molecular weight: | 57kDa/62kDa |
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