Western blot analysis of extracts of various cell lines, using PDPK1 antibody (A1665) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and PDPK1 knockout (KO) HCT116 cells, using PDPK1 antibody (A1665) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] PDPK1 Rabbit pAb
Catalog No:AB-A1665
Manufacturer:ABclonal
Synonyms:PDPK1;PDK1;PDPK2;PDPK2P;PRO0461
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] PDPK1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | PDPK1 (3-phosphoinositide dependent protein kinase 1) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity and insulin receptor binding. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1 (NP_112558.2). |
Gene ID: | 5170 |
Swiss prot: | O15530 |
Molecular weight: | 48kDa/50kDa/58kDa/60kDa/63kDa |
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