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Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP antibody (A19596) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of Jurkat cells, using PARP1 antibody (A19596) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody (A19596) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody (A19596) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using PARP1 antibody (A19596) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PARP1 antibody (A19596) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] PARP1 Rabbit mAb
Catalog No：AB-A19596
Manufacturer：ABclonal
Synonyms：ADPRT;ADPRT 1;ADPRT1;ARTD1;PARP;PARP-1;PPOL;pADPRT-1;PARP1;Cleaved PARP;PARP1
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] PARP1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human PARP1. |
| Gene ID: | 142 |
| Swiss prot: | P09874 |
| Molecular weight: | 113,89kDa |
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