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Western blot analysis of extracts of various cell lines, using P4HB antibody (A0692) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibody (A0692) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human lung cancer using P4HB antibody (A0692) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using P4HB antibody (A0692) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using P4HB antibody (A0692) at dilution of 1:150 (40x lens).
[KO Validated] P4HB Rabbit pAb
Catalog No：AB-A0692
Manufacturer：ABclonal
Synonyms：P4HB;CLCRP1;DSI;ERBA2L;GIT;P4Hbeta;PDI;PDIA1;PHDB;PO4DB;PO4HB;PROHB
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] P4HB Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2). |
| Gene ID: | 5034 |
| Swiss prot: | P07237 |
| Molecular weight: | 57kDa |
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