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Western blot analysis of extracts of various cell lines, using NUCB1 antibody (A3994) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and NUCB1 knockout (KO) 293T cells, using NUCB1 antibody (A3994) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
[KO Validated] NUCB1 Rabbit pAb
Catalog No：AB-A3994
Manufacturer：ABclonal
Synonyms：NUCB1;CALNUC;NUC
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] NUCB1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2). |
| Gene ID: | 4924 |
| Swiss prot: | Q02818 |
| Molecular weight: | 53kDa |
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