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Western blot analysis of extracts of various cell lines, using NT5E / CD73 antibody (A2029) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of Rat eye, using NT5E / CD73 antibody (A2029) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts from normal (control) and NT5E / CD73 Rabbit pAb knockout (KO) HCT116 cells, using NT5E / CD73 Rabbit pAb antibody (A2029) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human placenta using NT5E/CD73 antibody (A2029) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using NT5E/CD73 antibody (A2029) at dilution of 1:200 (40x lens).
[KO Validated] NT5E / CD73 Rabbit pAb
Catalog No：AB-A2029
Manufacturer：ABclonal
Synonyms：NT5E;CALJA;CD73;E5NT;NT;NT5;NTE;eN;eNT
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] NT5E / CD73 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E / CD73 (NP_002517.1). |
| Gene ID: | 4907 |
| Swiss prot: | P21589 |
| Molecular weight: | 57kDa/63kDa |
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