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Western blot analysis of extracts of various cell lines, using NF2 antibody (A0739) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts from normal (control) and NF2 knockout (KO) HeLa cells, using NF2 antibody (A0739) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 6s.
[KO Validated] NF2 Rabbit pAb
Catalog No：AB-A0739
Manufacturer：ABclonal
Synonyms：NF2;ACN;BANF;SCH;merlin
Applications：WB,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] NF2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1). |
| Gene ID: | 4771 |
| Swiss prot: | P35240 |
| Molecular weight: | 19-30kDa/59-72kDa |
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