Western blot analysis of extracts of various cell lines, using NDUFA5 antibody (A14751) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) 293T cells, using NDUFA5 antibody (A14751) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] NDUFA5 Rabbit pAb
Catalog No:AB-A14751
Manufacturer:ABclonal
Synonyms:NDUFA5;B13;CI-13KD-B;CI-13kB;NUFM;UQOR13
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] NDUFA5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1). |
Gene ID: | 4698 |
Swiss prot: | Q16718 |
Molecular weight: | 13kDa |
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