.jpg)
Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A3045) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
.jpg)
Western blot analysis of extracts of HeLa cells, using N-Cadherin antibody (A3045) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
.jpg)
Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A3045) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] N-Cadherin Rabbit pAb
Catalog No：AB-A3045
Manufacturer：ABclonal
Synonyms：CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
Applications：WB,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] N-Cadherin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin (NP_001783.2). |
| Gene ID: | 1000 |
| Swiss prot: | P19022 |
| Molecular weight: | 97kDa/99kDa |
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