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Western blot analysis of extracts from normal (control) and MSH6 knockout (KO) HeLa cells, using MSH6 antibody (A18063) at 1:300 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
[KO Validated] MSH6 Rabbit pAb
Catalog No：AB-A18063
Manufacturer：ABclonal
Synonyms：MSH6;GTBP;GTMBP;HNPCC5;HSAP;p160
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €425,00
| Product Name: | [KO Validated] MSH6 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. |
| Immunogen: | Recombinant protein of human MSH6 |
| Gene ID: | 2956 |
| Swiss prot: | P52701 |
| Molecular weight: | 119kDa/120kDa/137kDa/152kDa |
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