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Western blot analysis of extracts of various cell lines, using MEK1 antibody (A12687) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and MEK1 knockout (KO) HeLa cells, using MEK1 antibody (A12687) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded Rat brain using MEK1 antibody (A12687) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using MEK1 antibody (A12687) at dilution of 1:100 (40x lens).
[KO Validated] MEK1 Rabbit pAb
Catalog No：AB-A12687
Manufacturer：ABclonal
Synonyms：CFC3;MAPKK1;MEK1;MKK1;PRKMK1;MAP2K1
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] MEK1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK1 (NP_002746.1). |
| Gene ID: | 5604 |
| Swiss prot: | Q02750 |
| Molecular weight: | 40kDa/43kDa |
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