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Western blot analysis of extracts from normal (control) and MANF knockout (KO) 293T cells, using MANF antibody (A19923) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30S.
[KO Validated] MANF Rabbit pAb
Catalog No：AB-A19923
Manufacturer：ABclonal
Synonyms：MANF;ARMET;ARP
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] MANF Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. |
| Immunogen: | Recombinant protein of human MANF. |
| Gene ID: | 7873 |
| Swiss prot: | P55145 |
| Molecular weight: | 20kDa |
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