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Western blot analysis of extracts of various cell lines, using MANF Antibody (A13371) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
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Western blot analysis of extracts from normal (control) and MANF knockout (KO) 293T cells, using MANF antibody (A13371) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30S.
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Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] MANF Rabbit pAb (A13371) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] MANF Rabbit pAb (A13371) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] MANF Rabbit pAb (A13371) at dilution of 1:100 (40x lens).
[KO Validated] MANF Rabbit pAb
Catalog No：AB-A13371
Manufacturer：ABclonal
Synonyms：MANF;ARMET;ARP
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] MANF Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-182 of human MANF (NP_006001.4). |
| Gene ID: | 7873 |
| Swiss prot: | P55145 |
| Molecular weight: | 20kDa |
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