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Western blot analysis of extracts of various cell lines, using H2AFY antibody (A7045) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and macroH2A.1 Rabbit pAb knockout (KO) 293T cells, using macroH2A.1 Rabbit pAb antibody (A7045) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human kidney using macroH2A.1 antibody (A7045) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using macroH2A.1 antibody (A7045) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using macroH2A.1 antibody (A7045) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung cancer using macroH2A.1 antibody (A7045) at dilution of 1:100 (40x lens).
[KO Validated] macroH2A.1 Rabbit pAb
Catalog No：AB-A7045
Manufacturer：ABclonal
Synonyms：H2AFY;H2A.y;H2A/y;H2AF12M;MACROH2A1.1;mH2A1;macroH2A1.2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] macroH2A.1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2). |
| Gene ID: | 9555 |
| Swiss prot: | O75367 |
| Molecular weight: | 39kDa |
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