Western blot analysis of extracts from normal (control) and Lrwd1 knockout (KO) HeLa cells, using Lrwd1 antibody (A19843) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] Lrwd1 Rabbit pAb
Catalog No:AB-A19843
Manufacturer:ABclonal
Synonyms:LRWD1;CENP-33;ORCA;Lrwd1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] Lrwd1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic peptide of human Lrwd1. |
Gene ID: | 222229 |
Swiss prot: | Q9UFC0 |
Molecular weight: | 70kDa |
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