Western blot analysis of extracts of various cell lines, using LITAF antibody (A5469) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Western blot analysis of extracts from normal (control) and LITAF knockout (KO) HeLa cells, using LITAF antibody (A5469) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] LITAF Rabbit pAb
Catalog No:AB-A5469
Manufacturer:ABclonal
Synonyms:LITAF;PIG7;SIMPLE;TP53I7
Applications:WB,IHC,IP
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] LITAF Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget’s disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LITAF (NP_004853.2). |
Gene ID: | 9516 |
Swiss prot: | Q99732 |
Molecular weight: | 15kDa/17kDa/23kDa |
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