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Western blot analysis of extracts from normal (control) and LDHB knockout (KO) 293T cells, using LDHB antibody (A18096) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] LDHB Rabbit pAb
Catalog No：AB-A18096
Manufacturer：ABclonal
Synonyms：LDHB;HEL-S-281;LDH-B;LDH-H;LDHBD;TRG-5
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] LDHB Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human LDHB (NP_002291.1). |
| Gene ID: | 3945 |
| Swiss prot: | P07195 |
| Molecular weight: | 36kDa |
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