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Western blot analysis of extracts of various cell lines, using LC3B antibody (A11282) at 1:500 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts of 293T cells, using LC3B antibody (A11282) at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 1:100 (40x lens).
[KO Validated] LC3B Rabbit pAb
Catalog No：AB-A11282
Manufacturer：ABclonal
Synonyms：LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] LC3B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| Immunogen: | A synthetic peptide of human LC3B |
| Gene ID: | 81631 |
| Swiss prot: | Q9GZQ8 |
| Molecular weight: | 14kDa |
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