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Western blot analysis of extracts of Jurkat cells, using KEAP1 antibody (A17061) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Western blot analysis of extracts of Rat lung, using KEAP1 antibody (A17061) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Western blot analysis of extracts from normal (control) and KEAP1 knockout (KO) 293T cells, using KEAP1 antibody (A17061) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded rat ovary using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).
[KO Validated] KEAP1 Rabbit pAb
Catalog No：AB-A17061
Manufacturer：ABclonal
Synonyms：KEAP1;INrf2;KLHL19
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€110,00 – €425,00
| Product Name: | [KO Validated] KEAP1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
| Immunogen: | A synthetic peptide of human KEAP1. |
| Gene ID: | 9817 |
| Swiss prot: | Q14145 |
| Molecular weight: | 69kDa |
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