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Western blot analysis of extracts of various cell lines, using IκBα antibody (A11397) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.
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Western blot analysis of extracts from normal (control) and IκBα knockout (KO) 293T cells, using IκBα antibody (A11397) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
[KO Validated] IκBα Rabbit pAb
Catalog No：AB-A11397
Manufacturer：ABclonal
Synonyms：IKBA;MAD-3;NFKBI;IKB alpha;NFKBIA;EDAID2
Applications：WB,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] IκBα Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IκBα (NP_065390.1). |
| Gene ID: | 4792 |
| Swiss prot: | P25963 |
| Molecular weight: | 35kDa |
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