Western blot analysis of extracts of various cell lines, using IκBα antibody (A11168) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts from normal (control) and IκBα Rabbit pAb knockout (KO) HeLa cells, using IκBα Rabbit pAb antibody (A11168) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] IκBα Rabbit pAb
Catalog No:AB-A11168
Manufacturer:ABclonal
Synonyms:IKBA;MAD-3;NFKBI;IKB alpha;NFKBIA;EDAID2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] IκBα Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IκBα (NP_065390.1). |
Gene ID: | 4792 |
Swiss prot: | P25963 |
Molecular weight: | 35kDa |
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