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Western blot analysis of extracts of various cell lines, using HK2 antibody (A0994) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and HK2 knockout (KO) HeLa cells, using HK2 antibody (A0994) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human breast cancer using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
[KO Validated] HK2 Rabbit pAb
Catalog No：AB-A0994
Manufacturer：ABclonal
Synonyms：HK2;HKII;HXK2;hexokinase-2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HK2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HK2 (NP_000180.2). |
| Gene ID: | 3099 |
| Swiss prot: | P52789 |
| Molecular weight: | 102kDa |
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