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Western blot analysis of extracts of various cell lines, using HK1 Rabbit mAb (A0533) at 1：1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts from normal (control) and HK1 knockout (KO) 293T cells, using HK1 Rabbit mAb (A0533) at 1：1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat kidney using HK1 Rabbit mAb (A0533) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using HK1 Rabbit mAb (A0533) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using HK1 Rabbit mAb (A0533) at dilution of 1:100 (40x lens).
[KO Validated] HK1 Rabbit mAb
Catalog No：AB-A0533
Manufacturer：ABclonal
Synonyms：HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €605,00
| Product Name: | [KO Validated] HK1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016] |
| Immunogen: | A synthesized peptide derived from human Hexokinase 1 |
| Gene ID: | 3098 |
| Swiss prot: | P19367 |
| Molecular weight: | 102kDa |
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