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Western blot analysis of extracts from normal (control) and HIF1α knockout (KO) HeLa cells, using HIF1α antibody (A16873) at 1:1000 dilution.Both Hela cells and HIF1α knockout (KO) Hela cells were treated by Cobalt chloride (250uM) at 37℃ for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded rat ovary using HIF1α Rabbit pAb (A16873) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using HIF1α Rabbit pAb (A16873) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using HIF1α Rabbit pAb (A16873) at dilution of 1:100 (40x lens).
[KO Validated] HIF1α Rabbit pAb
Catalog No：AB-A16873
Manufacturer：ABclonal
Synonyms：HIF1A;HIF-1-alpha;HIF-1A;HIF-1alpha;HIF1;HIF1-ALPHA;MOP1;PASD8;bHLHe78
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] HIF1α Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
| Immunogen: | Recombinant protein of human HIF1α. |
| Gene ID: | 3091 |
| Swiss prot: | Q16665 |
| Molecular weight: | 82kDa/92kDa/95kDa |
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