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Western blot analysis of extracts of various cell lines, using HIF1AN/FIH1 antibody (A5466) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and HIF1AN/FIH1 Rabbit pAb knockout (KO) 293T cells, using HIF1AN/FIH1 Rabbit pAb antibody (A5466) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using HIF1AN/FIH1 antibody (A5466) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN/FIH1 antibody (A5466) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN/FIH1 antibody (A5466) at dilution of 1:100 (40x lens).
[KO Validated] HIF1AN/FIH1 Rabbit pAb
Catalog No：AB-A5466
Manufacturer：ABclonal
Synonyms：HIF1AN;FIH1
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HIF1AN/FIH1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2). |
| Gene ID: | 55662 |
| Swiss prot: | Q9NWT6 |
| Molecular weight: | 40kDa |
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