Western blot analysis of extracts of various cell lines, using HIF1AN/FIH1 antibody (A5466) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and HIF1AN/FIH1 Rabbit pAb knockout (KO) 293T cells, using HIF1AN/FIH1 Rabbit pAb antibody (A5466) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] HIF1AN/FIH1 Rabbit pAb
Catalog No:AB-A5466
Manufacturer:ABclonal
Synonyms:HIF1AN;FIH1
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] HIF1AN/FIH1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2). |
Gene ID: | 55662 |
Swiss prot: | Q9NWT6 |
Molecular weight: | 40kDa |
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