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Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Western blot analysis of extracts from normal (control) and HIF1α knockout (KO) HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (250uM) at 37℃ for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] HIF1α Rabbit pAb
Catalog No：AB-A11945
Manufacturer：ABclonal
Synonyms：HIF1A;HIF-1-alpha;HIF-1A;HIF-1alpha;HIF1;HIF1-ALPHA;MOP1;PASD8;bHLHe78
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] HIF1α Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-260 of human HIF1α (NP_851397.1). |
| Gene ID: | 3091 |
| Swiss prot: | Q16665 |
| Molecular weight: | 82kDa/92kDa/95kDa |
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