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Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A19571) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (A19571) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunofluorescence analysis of C6 cells using HDAC1 antibody (A19571) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using HDAC1 antibody (A19571) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded Rat testis using HDAC1 antibody (A19571) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse brain using HDAC1 antibody (A19571) at dilution of 1:100 (40x lens).
[KO Validated] HDAC1 Rabbit mAb
Catalog No：AB-A19571
Manufacturer：ABclonal
Synonyms：GON-10;HD1;RPD3;RPD3L1;HDAC1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HDAC1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human HDAC1. |
| Gene ID: | 3065 |
| Swiss prot: | Q13547 |
| Molecular weight: | 55kDa |
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