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Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (Glutathione Synthetase (GSS)) antibody (A14535) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells, using Glutathione Synthetase (Glutathione Synthetase (GSS)) antibody (A14535) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] Glutathione Synthetase (GSS) Rabbit pAb
Catalog No：AB-A14535
Manufacturer：ABclonal
Synonyms：GSS;GSHS;HEL-S-64p;HEL-S-88n
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human Glutathione Synthetase (Glutathione Synthetase (GSS)) (NP_000169.1). |
| Gene ID: | 2937 |
| Swiss prot: | P48637 |
| Molecular weight: | 40kDa/52kDa |
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