Western blot analysis of extracts of various cell lines, using FXN / Frataxin antibody (A16688) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and FXN / Frataxin knockout (KO) 293T cells, using FXN / Frataxin antibody (A16688) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
[KO Validated] FXN / Frataxin Rabbit pAb
Catalog No:AB-A16688
Manufacturer:ABclonal
Synonyms:FXN;CyaY;FA;FARR;FRDA;X25;frataxin
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] FXN / Frataxin Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin (NP_000135.2). |
Gene ID: | 2395 |
Swiss prot: | Q16595 |
Molecular weight: | 19kDa/21kDa/23kDa |
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