Western blot analysis of extracts from normal (control) and EXOC2 knockout (KO) HeLa cells, using EXOC2 antibody (A19948) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] EXOC2 Rabbit pAb
Catalog No:AB-A19948
Manufacturer:ABclonal
Synonyms:EXOC2;SEC5;SEC5L1;Sec5p
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] EXOC2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant protein of human EXOC2. |
Gene ID: | 55770 |
Swiss prot: | Q96KP1 |
Molecular weight: | 104kDa |
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