Western blot analysis of extracts of various cell lines, using eIF4EBP1 antibody (A1248) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 300s.
Western blot analysis of extracts from normal (control) and eIF4EBP1 knockout (KO) HeLa cells, using eIF4EBP1 antibody (A1248) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] eIF4EBP1 Rabbit pAb
Catalog No:AB-A1248
Manufacturer:ABclonal
Synonyms:4E-BP1;4EBP1;BP-1;PHAS-I;EIF4EBP1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] eIF4EBP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human eIF4EBP1 (NP_004086.1). |
Gene ID: | 1978 |
Swiss prot: | Q13541 |
Molecular weight: | 12kDa |
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