Western blot analysis of extracts of various cell lines, using EGFR antibody (A2069) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of Rat brain, using EGFR antibody (A2069) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (A2069) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] EGFR Rabbit pAb
Catalog No:AB-A2069
Manufacturer:ABclonal
Synonyms:EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] EGFR Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human EGFR (NP_005219.2). |
Gene ID: | 1956 |
Swiss prot: | P00533 |
Molecular weight: | 44kDa/69kDa/77kDa/134kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-A0951
Manufacturer:ABclonal
Synonyms:FOX-3;FOX3;HRNBP3;NEUN;NeuN;RBFOX3
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €1.170,00
Catalog No:AB-A0783
Manufacturer:ABclonal
Synonyms:NBN;AT-V1;AT-V2;ATV;NBS;NBS1;P95;nibrin
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0561
Manufacturer:ABclonal
Synonyms:ANOP3;MCOPS3;SOX2;SRY-box 2
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0533
Manufacturer:ABclonal
Synonyms:HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €605,00
Catalog No:AB-A0290
Manufacturer:ABclonal
Synonyms:CDKN1B;CDKN4;KIP1;MEN1B;MEN4;P27KIP1;p27 KIP 1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0281
Manufacturer:ABclonal
Synonyms:CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00