Western blot analysis of extracts of various cell lines, using EEF2K antibody (A5404) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 40s.
Western blot analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody (A5404) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] EEF2K Rabbit pAb
Catalog No:AB-A5404
Manufacturer:ABclonal
Synonyms:EEF2K;HSU93850;eEF-2K
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] EEF2K Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1). |
Gene ID: | 29904 |
Swiss prot: | O00418 |
Molecular weight: | 82kDa |
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