Western blot analysis of extracts of various cell lines, using EDF1 antibody (A2283) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and EDF1 Rabbit pAb knockout (KO) NIH/3T3 cells, using EDF1 Rabbit pAb antibody (A2283) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] EDF1 Rabbit pAb
Catalog No:AB-A2283
Manufacturer:ABclonal
Synonyms:EDF1;CFAP280;EDF-1;MBF1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] EDF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1). |
Gene ID: | 8721 |
Swiss prot: | O60869 |
Molecular weight: | 15kDa/16kDa |
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