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Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 antibody (A19679) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Western blot analysis of extracts of various cell lines, using DNMT1 antibody (A19679) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Immunofluorescence analysis of C6 cells using DNMT1 antibody (A19679). Blue: DAPI for nuclear staining.
[KO Validated] DNMT1 Rabbit mAb
Catalog No：AB-A19679
Manufacturer：ABclonal
Synonyms：ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] DNMT1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Immunogen: | A synthesized peptide derived from human DNMT1. |
| Gene ID: | 1786 |
| Swiss prot: | P26358 |
| Molecular weight: | 183kDa |
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