Western blot analysis of extracts from normal (control) and DNAJC2 knockout (KO) 293T cells, using DNAJC2 antibody (A19954) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] DNAJC2 Rabbit pAb
Catalog No:AB-A19954
Manufacturer:ABclonal
Synonyms:DNAJC2;MPHOSPH11;MPP11;ZRF1;ZUO1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] DNAJC2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. |
Immunogen: | Recombinant protein of human DNAJC2. |
Gene ID: | 27000 |
Swiss prot: | Q99543 |
Molecular weight: | 65kDa/71kDa |
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