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Western blot analysis of extracts from normal (control) and DNA Ligase IV knockout (KO) HeLa cells, using DNA Ligase IV antibody (A18050) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
[KO Validated] DNA Ligase IV Rabbit pAb
Catalog No：AB-A18050
Manufacturer：ABclonal
Synonyms：LIG4;LIG4S
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] DNA Ligase IV Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human DNA Ligase IV (NP_002303.2). |
| Gene ID: | 3981 |
| Swiss prot: | P49917 |
| Molecular weight: | 103kDa |
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