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Western blot analysis of extracts from normal (control) and Cyclin B1 knockout (KO) HeLa cells, using Cyclin B1 antibody (A19037) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (A19037) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] Cyclin B1 Rabbit mAb (A19037) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Cyclin B1 antibody (A19037). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (A19037) at a dilition of 1:1000.
[KO Validated] Cyclin B1 Rabbit mAb
Catalog No：AB-A19037
Manufacturer：ABclonal
Synonyms：CCNB;CCNB1;Cyclin B1;cyclin B1
Applications：WB,IHC,IP
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €1.170,00
| Product Name: | [KO Validated] Cyclin B1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human Cyclin B1 |
| Gene ID: | 891 |
| Swiss prot: | P14635 |
| Molecular weight: | 55kDa |
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